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You are investigating a mutation on a steroid receptor that confers a destructive bone disease. You express the mutant protein in a cell, and you examine the gene expression effects by adding the steroid ligand. You noticed that the gene expression profile changes in the cell expressing the mutant protein. You further demonstrate that the steroid hormone ligand binds to the mutant receptor with the same affinity as the normal receptor and using fluorescently labelled protein you also show the mutant protein can be found in the nucleus.Where do you think the mutation affects the steroid receptor?

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