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XX : Exons et introns dans un gène. Règle AG – GT. Tous les exons sauf le premier et le dernier vont avoir juste avant de démarrer (dans la partie intronique) deux bases AG. À la fin de la séquence exonique on a toujours deux bases GT. Pour le premier exon pas de AG en 5', le dernier exon n'a pas le GT en 3'. Tous les autres exons ont cette règle AG-GT. S’il y a une mutation sur ces bases c'est une altération de l'épissage. Indispensable pour l'excision de l'intron quand il y a maturation de l'ARNm (le lasso ne se forme pas et on ne peut pas produire un ARNm normal).

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