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Why is it important to make sure the solution is basic with a pH of at least 10 before extracting sodium benzoate?

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Which of the following statements is TRUE?DNA polymerase incorporates new nucleotides into the growing DNA molecule by forming hydrogen bonds between complementary nucleotides on opposite strands of the double helix. Only the lagging strand requires the enzyme primase. Once helicase has initially opened up the DNA double helix, it dissociates from the molecule allowing single strand binding proteins and DNA polymerase to bind. Primase, an RNA polymerase, is capable of synthesizing RNA without attaching the incoming nucleotide to a pre-existing, free, 3' hydroxyl group. Only the lagging strand requires the enzyme primase.
Epinephrine inhibits salivary gland activity and muscle cell contraction in the airways, yet stimulates glycogen release in muscle cells and heart muscle cell contraction. How could one hormone have opposing effects on different organs? Only responsive tissues are exposed to the epinephrine in the blood stream. Cells in tissues that are not responsive contain enzymes that break down epinephrine when it enters the cell. Cells in responsive tissues have signal transduction pathways. Cells in the different tissues would have epinephrine receptors coupled to different signaling pathways. Cells in the tissues that are suppressed do not express an epinephrine receptor.
A mutation in a G-protein prevents the alpha-subunit from dissociating from the beta/gamma-subunit. What effect will this have on the pathway in which the G-protein is involved? The pathway will be under-activated. The pathway will be activated even in the absence of a signal. The pathway will be over-activated.

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