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The cells of beetroot contain a red pigment. A student investigated the effect of temperature on the loss of red pigment from beetroot. He put discs cut from beetroot into tubes containing water. He maintained each tube at a different temperature. After 25 minutes, he measured the percentage of light passing through the water in each tube.The student put the same volume of water in each tube.Explain why it was important that he controlled this experimental variable. (2)

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