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PCR is normally carried out using the enzyme Taq DNA polymerase. The enzyme wasoriginally extracted from the bacterium Thermus aquaticus. This bacterium was found inMushroom Spring, one of the hot springs in Yellowstone National Park in the USA.Taq DNA polymerase is now obtained from genetically modified Escherichia coli that carriesthe Taq DNA polymerase gene.The optimum temperature of this enzyme is 75 - 80oC.After 40 minutes at 95oC the activity of the enzyme is reduced by half. This means that thehalf-life of this enzyme at 95oC is 40 minutes.(i) Suggest and explain why Taq DNA polymerase is now obtained from geneticallymodified E. coli. [2]

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Choose the best way to connect sentences 11 and 12 with a coordinating conjunction.Select one:a. The first pilgrim the narrator introduces is the Knight, and the treatment of this nobleman is a good example of how the narrator combines the general and the particular.b. The first pilgrim the narrator introduces is the Knight, but the treatment of this nobleman is a good example of how the narrator combines the general and the particular.c. The first pilgrim the narrator introduces is the Knight, yet the treatment of this nobleman is a good example of how the narrator combines the general and the particular.d. The first pilgrim the narrator introduces is the Knight, so the treatment of this nobleman is a good example of how the narrator combines the general and the particular.

The Periplus offers key insights into the military techniques of Ashoka. slave trade between Arabia and East Africa. Indian Ocean trade. iron-ore mining techniques of the firstt century C.E. manufacturing techniques of Indian cotton goods.

Dans quelle science la méthode expérimentale a t'elle été mise au point ?

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