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l'ecologie de la santé, avancer mar hippocrate. Plaide pour intégré la medecine, le soin de l'humain, des non humains et de la nature, a l'heure de la transition écologique

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*10.30 Chapter 9 presented a description of how DNA microarray analysis was used to characterize changes to the transcriptome during yeast sporulation. That analysis found that more than 1,000 yeast genes showed significant changes in mRNA levels during sporulation, identifiedat least seven distinct temporal patterns of gene induction, and provided insights into the functions of many orphan genes. It is important to confirm findingsfrom microarray analyses using independent methods. How would you confirm independently that three orphan genes display altered expression during yeast sporulation?
*10.24 Imagine that you have cloned the structural gene for an enzyme that functions in the biosynthesis of catecholaminesin the adrenal gland of rats. How could you use this cloned DNA as a probe to determine whether this same gene functions in the rat brain?
*10.33 DNA was prepared from small samples of white blood cells from a large number of people. These DNAs were individually digested with EcoRI, subjected toelectrophoresis and Southern blotting, and the blot was probed with a radioactively labeled cloned human sequence. Ten different patterns were seen among all of the samples. The following figure shows the results seen in ten individuals, each of whom is representative of a differentpattern. Table herea. Explain the hybridization patterns seen in the 10 representative individuals in terms of variation in EcoRI sites.b. If the individuals whose DNA samples are in lanes 1 and 6 on the blot were to produce offspring together, what bands would you expect to see in DNA samplesfrom these offspring?

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