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For the purpose of human gene therapy, DeWitt et al. want to avoid genetically engineering a patient's cells to express Cas9 and sgRNA. Instead, they chose to treat a patient's cells with purified recombinant Cas9 protein and sgRNA.What is the most direct and easiest way to make a copy of the Cas9 gene, which is naturally found in a bacterium called Streptococcus pyogenes? The entire genome of S. pyogenes has been sequenced and is publicly available in Genbank.
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