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Con relacion a los test que presentan una alta sensibildad, es cierto que:

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- a spiral organism, has variable number (7-11) of endoflagella- Are highly motile.- Stains readily with aniline dyes and by silver impregnation techniques- Cultivation- grows very slowly in a complex liquid media (isolated from ECM skin lesions or ticks) - low diagnostic yield, it is seldom used

- Outer surface proteins: OspA and F- (OspA and OspB in ticks)- The transmission of B.burgdorferi to humans is by injection of the organism in tick saliva or by regurgitation of the tick's midgut contents. Attachment of the tick for 24-48 h is necessary before transmission of B.burgdorferi → B.burgdorferi adheres to proteoglycans on host cells, this is mediated by a borrelial glycosaminoglycan receptor → After injection by the tick, the organism migrates out from the site, producing the characteristic skin lesion → Dissemination occurs by lymphatics or blood to other skin and musculoskeletal sites and many other organs → Lyme disease, like other spirochetal diseases, occurs in stages with early and late manifestations- Lyme disease:1. Erythema chronicum migrans (ECM)a. A unique skin lesion is Erytema chronicum migrans (ECM), which occurs at the tick bite site.2. Neurologic/Cardiologic manifestationa. Occurs weeks to months later and includes arthralgia and arthritis; neurologic manifestations with meningitis; and cardiac disease with conduction defects and myopericarditis.3. Arthritisa. Begins months to years later with chronic skin, nervous system,or joint involvement.

- long, helically coiled (6-25 coils), spiral or corkscrew-shaped- T.pallidum has an outer sheath or glycosaminoglycan coating.- Inside the sheath is the outer membrane (OM) Endoflagella (axial filaments) are the flagella-like organelles in the PPS encased by the OM. Gr- with no classical LPS structure. Reproduce by transverse fission.- Do not stain well with aniline dyes (silver impregnation method can be used)

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