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Genetics

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step 1 of elongationbinding of second aminoacyl _______________ into A site
___________ structurally resembles EF-Tu bound to tRNA
___________ forces the translocation step during elongation by fitting in _____ site
____________ more abundant protein in E. coli
Upon GTP hydrolysis and release of EF-Tu, ribosome rotates ____ A so that amino acid linked to tRNA in A site abuts amino acid on tRNA in ___ site
EF-Tu hydrolyzes GTP once correct _______ is found which pushes amino acid into ___ site.
_______ hydrolysis by EF-G triggers its _____________ to change, which moves peptidyl-tRNA from A to P
Step 2 of elongationpeptide bond formation between _____________ in the ___ site and __ site transferring the growing polypeptide to the tRNA in the ___ site
peptide bond formationNew incoming amino acid acts as _____________ and cleaves the ____________ bond made with the _____________ to create a _____________ bond
EF-Tu will only bring tRNA in when bound to ______
EF protein that helps with binding, making sure correct charged tRNA is loaded into A site
Translation Initiation in eukaryoteseIF2-GTP brings in ___________
Translation Initiation in eukaryotesViruses:RNA 2D structure enables recruitment of either __________ of directly the ribosome
Translation Initiation in eukaryotesIRES located ____________ of start codon
Translation Initiation in bacteriaMet-tRNA^fMet accompanied by ______ basepairs with ______ codon
Translation Initiation in eukaryotes60 + 40 = _____
Immediately following the first peptide bond formation, the ribosome moves _____ codon towards ____'-end of mRNA
Translation Initiation in eukaryotesOnce the start codon base-pairs with tRNAi anti-codon, series of ________ hydrolyses => release of all eIFs except ____________ (still bond to cap) and assembly of ______________ ribosome subunit
Translation Initiation in eukaryotes______________ sequence in eukaryotic mRNAs is analogous to shine-dalgarno
Translation Initiation in eukaryotes______ particle scans mRNA 5' to 3' to the first __________

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