English Questions
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Which of the following is NOT a feature of the Cas9 protein?a. cleaves target sequencesb. binds to guide RNAc. can be modified to create custom activators or repressorsd. recognizes specific target sequences
HSPA8 is a chaperone protein that binds to hydrophobic regions of newly synthesized (nascent) proteins in an ATP-dependent manner. Hydrolysis of ATP by HSPA8 is likely required in order for the nascent protein to:
In which scenario would the clustered regularly interspaced short palindromic repeats (CRISPR) system provide valuable information to test a hypothesis?a. inverting a gene or DNA sequence to assay altered expression phenotypeb. sequencing a spontaneous mutation that results in a phenotype of interestc. assaying gene-expression changes under different environmental conditiod. precisely knocking out a specific gene or gene region to test its function
Place the following events of RNA splicing into the correct sequence:1. Formation of a 2' to 5' phosphodiester bond between the branch point and 5' end of the intron2. the first transesterification reaction, catalyzed by U2 and U63. recruitment of U3, U4, and U5 to the preRNA4. binding of SF1 to the branch point A5. joining of the two exons via a 3' to 5' phosphodiester bond
A DNA sequencing (Sanger sequencing) reaction contains which of the followinga. dideoxyribonucleotide triphosphatesb. deoxyribonucleotide triphosphatesc. DNA polymerased. all of the above
All of the following specialized sequences are needed by eukaryotic chromosomes EXCEPT: a. a centromere for mitotic segregationb. a kinetochore for mitotic segregationc. an origin of replicationd. telomeres at the ends of chromosomese. all of these are required DNA sequences
Which of the following is NOT true of cDNA libraries?a. they contain the exons of genesb. they contain the introns of genesc. they are best used for simple organismsd. they are made using an organism's mRNAe. they require the use of reverse transcriptase
Telomerase:a. contains its own RNA templateb. is a reverse transcriptasec. extends one strand of the telomered. is responsible for helping to maintain chromosome sizee. all of the abovef. none of the above
What technique is used to visualize the localization of the expression of a particular gene of interest via its mRNA?
Which of the following is a segment of DNA?a. enhancerb. activatorc. transcription factord. repressor
Put the following steps for screening an oligonucleotide array for patterns of gene expression in the correct order:1. the activated genes in each sample are identified 2. a scanner detects patterns of hybridization3. pools of cDNAs are incubated with the array under hybridization conditions4. total mRNA is isolated from each sample to be analyzed5. the mRNAs are converted to flourescently labeled cDNAs
Plasmid cloning vectors have replication _____, instead of replication forks.
What is a difference between the two CRISPR-Cas9 editing mechanisms: homology-directed repair and non homologous end joining?
Which of the following regulate protein activity in a cell?a. change in steady state levelsb. binding of inhibitors or activatorsc. change in cellular protein locationd. all of the above
Which base(s) form a Watson-Crick base pair with Guanine?
Which of the following is NOT a key feature of a plasmid cloning vector?a. replication originb. antibiotic resistance genec. polylinkerd. inverted repeats
What is used to cleave a vector at the cloning site?
Okazaki fragments are short sequences of DNA nucleotides that create the _____ _____ during DNA replication.
A reporter construct in which the green fluorescent protein (GFP) gene is fused to gene X is best used to:
Which of the following types of mobile DNA elements typically do NOT pass through an RNA intermediate stage while "jumping" from one genomic location to another?a. DNA transposonsb. retrotransposonsc. LINESd. SINES